Miguel A. Arroyo, Michael Hoelscher, Eric Sanders-Buell, Karl-Heinz Herbinger, Eleuter Samky, Leonard Maboko, Oliver Hoffmann, Merlin R. Robb, Deborah L. Birx, and Francine E. McCutchan
HIV-1 is endemic in Tanzania where three different subtypes, A, C, and D, have been identified. Information on HIV-1 genetic diversity is crucial to define requirements for an effective vaccine, in regions where HIV-1 vaccine trials are planned. To define the subtype distribution of HIV-1 in the Mbeya region of southwest Tanzania, peripheral blood mononuclear cells (PBMC) and plasma were obtained from 36 discarded HIV seropositive blood units. Multiregion hybridization assay (MHA) was performed on both PBMC DNA and plasma RNA to determine the subtype distribution. Twenty virtually full-length HIV-1 sequences were amplified from the extracted DNA, sequenced, and phylogenetically analyzed. Subtype distribution......
Published at: 2004-08-25
Jordan-Harder, Brigittea; Maboko, Leonardb; Mmbando, Donanb; Riedner, Gabrielec; Nägele, Elisabethd; Harder, Juana; Küchen, Volkere; Kilian, Alberta; Korte, Rolfa; Sonnenburg, Frank vd
To describe the evolution of the HIV-1 epidemic among women attending antenatal clinics in four different geographic areas within two rural regions with differing intervention intensity in south-west Tanzania.
Published at: 2004-01-23